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- Whatman Spartan Filter 13 RC/PP 0.45µm Pack of 500
Whatman Spartan Filter 13 RC/PP 0.45µm Pack of 500
- £844.00 excl. VAT
- £1,012.80incl. VAT
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Print PageThe SPARTAN® syringe filters ensure reproducible results from the filtration of organic and aqueous solutions in HPLC. For batch-to-batch consistency, the SPARTAN range of filters are tested and certified for the absence of UV-absorbing substances at wavelengths of 210 and 254 nm with water, methanol and acetonitrile.
Features:
Ready-to-use filter unit with a hydrophilic, low protein-binding membrane made of regenerated cellulose
- Excellent chemical resistance against the standard aqueous and organic HPLC solvents
- SPARTAN syringe filters are tested and certified for the absence of UV- absorbing substances at wavelengths of 210 and 254nm with water, methanol and acetonitrile
- 13mm diameter with Mini-Tip
- 13mm diameter with extremely low dead volume <10µl
Benefits:
- Versatile: Use for any application requiring a chemically resistant, hydrophilic, low protein-binding membrane
- Documented batch-to-batch quality and consistency ensure reproducible results
- 13mm diameter with Mini-tip outlet is ideal for filtration into very small sample bottles
Applications:
- Filtration of organic and aqueous solutions in HPLC with reproducible results
- Purification of aqueous and organic solutions
- Filtration of protein solutions
Guide to…Syringe Filter Properties
Identifying the filter material most suitable for your application based upon the nature of the liquid or gas you are filtering will ensure the best performance of your filter. Each syringe filter contains a membrane filter of a set diameter and plastic housing with luer connections used for connecting to syringes (or in-line hosing systems).
The membrane filter material has differing chemical and physical properties such as hydrophobicity, flow rate, extractables and protein retention making them suited to differing applications. Once you have selected the most compatible filter material, you will need to consider what pore size is most appropriate for your application and what diameter filter is suitable for your sample volume.
Membrane Material
| Membrane Material | Protein binding | Hydrophilic/Hydrophobic | Chemical resistance | Applications |
| Hydrophilic PTFE | Low | Hydrophilic | High | Purification of HPLC organic and solvent/aqueous solutions |
| Hydrophobic PTFE | Low | Hydrophobic | High | Filtration of solvents |
| PES | Low | Hydrophilic | Low/Medium | Purification of tissue culture solutions, buffers |
| PVDF | Low | Hydrophilic | Medium | Filtration of protein solutions |
| NYL | Medium | Hydrophilic | Medium | Filtration of aqueous and solvent/aqueous mixtures |
| CA | Low | Hydrophilic | Low | Filtration of protein solutions |
| CN | High | Hydrophilic | Low | Filtration of aqueous solutions where protein binding is not a concern and microbioloigical applications. |
| RC | Low | Hydrophilic | Medium | Filtration of aqueous and organic solvents and protein recovery applications. |
Filter diameter
High particulate loaded samples with tend to block the pores in the membrane more quickly so the total volume you can filter through a certain diameter syringe filter will vary greatly. Please take the below figures as a guide only.
- 13mm diameter - for samples <10ml
- 25mm diameter - for samples <100ml
- 33mm diameter - for samples <250ml
For samples that are very particle ladden, it is recommened that you try pre-filtering your sample. Pre-filtering can be achieved with a number of different filters, such as glass microfibre. Some syringe filters come with an in-built pre-filter.
