One of the major applications for the PTFE membrane is the clarification of corrosives, solvents and aggressive fluids. This includes the important requirement in HPLC analysis for sample filtration where any solid particles can cause permanent damage to the column. The 0.5µm pore size is normally used. Air and gas sterilization make use of the hydrophobic characteristics of PTFE membranes and their ability to stop aqueous aerosols. Usual pore sizes are 0.2µm and 0.5µm. Sterile venting of vacuum manifolds, fermentation vessels and sterile filtrate tanks and containers utilize PTFE 0.2µm membranes.
The WTP membranes use a polypropylene grid as the support material
Identifying the filter material most suitable for your application based upon the nature of the liquid or gas you are filtering will ensure the best performance of your filter. Each membrane filter material has differing chemical and physical properties such as hydrophobicity, flow rate, extractables and protein retention making them suited to differing applications. Once you have selected the most compatible filter material, you will need to consider what pore size is most appropriate.
Membrane Material | Protein binding | Hydrophilic/Hydrophobic | Chemical resistance | Applications |
Hydrophilic PTFE | Low | Hydrophilic | High | Purification of HPLC organic and solvent/aqueous solutions |
Hydrophobic PTFE | Low | Hydrophobic | High | Filtration of solvents |
PES | Low | Hydrophilic | Low/Medium | Purification of tissue culture solutions, buffers |
PVDF | Low | Hydrophilic | Medium | Filtration of protein solutions |
NYL | Medium | Hydrophilic | Medium | Filtration of aqueous and solvent/aqueous mixtures |
CA | Low | Hydrophilic | Low | Filtration of protein solutions |
CN | High | Hydrophilic | Low | Filtration of aqueous solutions where protein binding is not a concern and microbioloigical applications. |
RC | Low | Hydrophilic | Medium | Filtration of aqueous and organic solvents and protein recovery applications. |