The Anopore„¢ inorganic membrane (Anodisc„¢) is well suited for a wide range of laboratory filtration applications. This novel material has a precise, nondeformable honeycomb pore structure with no lateral crossovers between individual pores, that filters at precisely the stated cut-off, allowing no larger sized particles to pass through the membrane. The Anopore inorganic membrane is composed of a high purity alumina matrix that is manufactured electrochemically. The membrane also exhibits low protein binding, has minimal autofluorescence, is nontoxic, and supports cellular growth.
The precise pore structure and narrow pore size distribution of the Anopore membrane ensure a high level of particle removal efficiency. Microorganisms and particulate material are captured on the surface of the membrane for subsequent analysis by light or electron microscopy. When wet, the membrane is virtually transparent, which means that retained particles do not need to be transferred to another surface before microscopic examination.
The membrane is hydrophilic and is compatible with most solvents and aqueous material. No monomers, plasticizers, adhesives, surfactants or wetting agents are used in the manufacturing process, which eliminates sample contamination and ensures low protein binding and minimal loss of sample.
The Anopore membrane is supplied in the form of Anodisc membrane filters. The membrane is peripherally bonded to an annular polypropylene ring (except the 13mm diameter disc) for ease of handling and is suitable for both vacuum and pressure filtration.
Anopore is available in 3 nominal pore sizes: 0.02µm, 0.1µm, and 0.2µm, and in 3 diameters: 13mm, 25mm, and 47mm.
Identifying the filter material most suitable for your application based upon the nature of the liquid or gas you are filtering will ensure the best performance of your filter. Each membrane filter material has differing chemical and physical properties such as hydrophobicity, flow rate, extractables and protein retention making them suited to differing applications. Once you have selected the most compatible filter material, you will need to consider what pore size is most appropriate.
Membrane Material | Protein binding | Hydrophilic/Hydrophobic | Chemical resistance | Applications |
Hydrophilic PTFE | Low | Hydrophilic | High | Purification of HPLC organic and solvent/aqueous solutions |
Hydrophobic PTFE | Low | Hydrophobic | High | Filtration of solvents |
PES | Low | Hydrophilic | Low/Medium | Purification of tissue culture solutions, buffers |
PVDF | Low | Hydrophilic | Medium | Filtration of protein solutions |
NYL | Medium | Hydrophilic | Medium | Filtration of aqueous and solvent/aqueous mixtures |
CA | Low | Hydrophilic | Low | Filtration of protein solutions |
CN | High | Hydrophilic | Low | Filtration of aqueous solutions where protein binding is not a concern and microbioloigical applications. |
RC | Low | Hydrophilic | Medium | Filtration of aqueous and organic solvents and protein recovery applications. |