Eppendorf µCuvette® G1.0

Eppendorf µCuvette® G1.0

Eppendorf µCuvette® G1.0. Microvolume measuring cell for Eppendorf BioPhotometer® and BioSpectrometer® - 6138000018

About the range

The Eppendorf µCuvette G1.0 microvolume measuring cell is a high-quality cuvette made of aluminum and quartz glass. It is the perfect tool for measuring high concentrations in small volumes.

With a fixed optical path length of only 1mm, the µCuvette G1.0 features a light path that is ten times shorter than in standard cuvettes. This allows nucleic acid and protein concentrations to be measured with high reproducibility in a much higher concentration range without prior dilution.

Due to the hydrophobic coating on the quartz glass, only 1.5µl nucleic acid or 3µl protein sample is required for precise formation of the liquid column. Self-absorption of the µCuvette G1.0 is very low, meaning that the entire measuring range of the photometer can be used. Furthermore, 5µl of sample solution can be used for specific fluorometric assays saving reagents.

In general, the lower the concentration of a sample, the longer the optical path length should be. The same physical model (Beer´s Lambert Law) claims, if the concentration of a sample is very high, the light path length being used should be shortened to ensure enough light can pass through the sample to reach the detector.

Microvolume measurements with light path lengths equal or shorter than 1mm are recommended for concentrations above 25ng/µl dsDNA.

Features:

• Microvolume measuring cell for measuring 1.5 - 10µl sample volumes
• Concentration determination of nucleic acids and proteins, amongst other target analytes
• Measurement of highly concentrated samples without prior dilution
• Suitable for fluorescence assays
• Low self-absorption (≤0.05A at 260nm)
• Hydrophobic surface coating on quartz glass for precise formation and positioning of the sample volume
• Simple cleaning to minimize the risk of sample carry-over
• A defined angle for folding the sides of the cuvette guarantees reproducible handling
• One-component hinged design prevents the loss of individual parts
• Exclusively available for use with Eppendorf BioPhotometer and Eppendorf BioSpectrometer