Eppendorf BioPhotometer® D30 Reference Filter Set

Eppendorf BioPhotometer® D30 Reference Filter Set

Eppendorf BioPhotometer® D30 Reference Filter Set - filter set for verifying photometric accuracy and wavelength systematic error (according to NIST®) - 6133928004

About the range

The Eppendorf BioPhotometer D30 is the 3rd generation of Eppendorf spectrophotometers to become an established standard in the life sciences.

Routine pre-defined wavelengths are easily selectable, allowing for quick and simple programming. Users are guided through every step of the assay with short, simple on-screen instructions.

The ultra-stable Xenon flash lamp doesn't require any warm up time and a measurement typically takes 5 seconds. Raw and processed data are clearly presented on the large color screen and can be exported directly from the instrument in a variety of common formats.

Eppendorf disposable UVette^® cuvettes enable measurements with as little as 50µl of sample and the Eppendorf µCuvette^® G1.0 reduces this further to 1.5µl of sample.

Nucleic acid purification is a major application in molecular laboratories. Purity and homogeneity of the sample are important considerations for subsequent applications. In practice, nucleic acids are purified with the help of commercially available kits which allow for the separation of most cellular components. However, the presence of proteins or other organic components in the eluate may not be entirely eliminated.

When purifying nucleic acid without a kit, additional contamination risks are posed by the chemicals used in the process, e.g. phenol or ethanol from a phenol/chloroform extraction. To ensure minimum contamination of the sample, it is essential to verify its purity by photometric measurements.

The ratios of the absorbance values at the wavelengths 230nm, 260nm and 280nm (260nm / 230nm and 260nm / 280nm), provide a clear picture of the purity of a nucleic acid sample. The following values represent a pure DNA sample:

A260 / A230 ≥ 2.0
A260 / A280 = 1.8 - 2.0

Features:

• Filter traceable to NIST^®
• Filter recertification via Eppendorf
• Inspection protocol preprogrammed in device
• Verification of wavelength accuracy and precision at 260nm and 280nm
• Verification of the photometric accuracy and precision for selected wavelengths
• Contains 6 filters (1 blank filter, 2 filters for checking UV/Vis wavelength systematic error and precision, 3 filters for photometric verification)

Applications:

• Nucleic acid quantification
• Direct protein quantification (UV 280nm)
• Microvolume measurement via µCuvette G1.0 for highly concentrated samples
• Bacteria growth measurement (OD 600)
• Colorimetric assays for protein quantification, e.g. BCA, Bradford, Lowry
• 340nm: endpoint assays using NADPH or NAPH
• 405nm: endpoint assays using para-nitrophenol
• 490nm: cytotoxicity endpoint assay
• Transmission measurement