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- Whatman Spartan Filter 30 RC/PP 0.45µm Pack of 50
Whatman Spartan Filter 30 RC/PP 0.45µm Pack of 50
- £142.04 excl. VAT
- £170.45incl. VAT
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Print PageWhatman Spartan syringe filters for HPLC.
- Hydrophilic, low protein binding regenerated cellulose
- PP housing
- Female luer lock inlet, luer slip outlet
- 30mm diameter
- 0.45µm pore size
- Pack of 50
Whatman SPARTAN syringe filters from Cytiva provide reproducible filtration for ultra-high performance liquid chromatography (UHPLC) and high performance liquid chromatography (HPLC) sample preparation and analysis.
- Hydrophilic membrane made of regenerated cellulose fiber exhibits low protein binding membrane
- Disposable filter unit with Polycarbonate housing
- Excellent chemical resistance to common aqueous and organic solvents, so recommended for HPLC method sample preparation
- Tested and certified for the absence of UV-absorbing substances at wavelengths of 210 and 254 nm with water, methanol, and acetonitrile
- Documented batch-to-batch quality and consistency, ensuring reproducible results
- Low dead volume
In addition to their use as HPLC syringe filters, SPARTAN filters can be used in capillary gel electrophoresis, protein sample ultrafiltration, UV/VIS analysis, and any application requiring a chemically resistant, low protein binding, hydrophilic membrane in a polycarbonate filter housing.
Guide to…Syringe Filter Properties
Identifying the filter material most suitable for your application based upon the nature of the liquid or gas you are filtering will ensure the best performance of your filter. Each syringe filter contains a membrane filter of a set diameter and plastic housing with luer connections used for connecting to syringes (or in-line hosing systems).
The membrane filter material has differing chemical and physical properties such as hydrophobicity, flow rate, extractables and protein retention making them suited to differing applications. Once you have selected the most compatible filter material, you will need to consider what pore size is most appropriate for your application and what diameter filter is suitable for your sample volume.
Membrane Material
| Membrane Material | Protein binding | Hydrophilic/Hydrophobic | Chemical resistance | Applications |
| Hydrophilic PTFE | Low | Hydrophilic | High | Purification of HPLC organic and solvent/aqueous solutions |
| Hydrophobic PTFE | Low | Hydrophobic | High | Filtration of solvents |
| PES | Low | Hydrophilic | Low/Medium | Purification of tissue culture solutions, buffers |
| PVDF | Low | Hydrophilic | Medium | Filtration of protein solutions |
| NYL | Medium | Hydrophilic | Medium | Filtration of aqueous and solvent/aqueous mixtures |
| CA | Low | Hydrophilic | Low | Filtration of protein solutions |
| CN | High | Hydrophilic | Low | Filtration of aqueous solutions where protein binding is not a concern and microbioloigical applications. |
| RC | Low | Hydrophilic | Medium | Filtration of aqueous and organic solvents and protein recovery applications. |
Filter diameter
High particulate loaded samples with tend to block the pores in the membrane more quickly so the total volume you can filter through a certain diameter syringe filter will vary greatly. Please take the below figures as a guide only.
- 13mm diameter - for samples <10ml
- 25mm diameter - for samples <100ml
- 33mm diameter - for samples <250ml
For samples that are very particle ladden, it is recommened that you try pre-filtering your sample. Pre-filtering can be achieved with a number of different filters, such as glass microfibre. Some syringe filters come with an in-built pre-filter.
