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- Whatman Puradisc 25 Nylon 0.45µm Pack of 50
Whatman Puradisc 25 Nylon 0.45µm Pack of 50
- £119.57 excl. VAT
- £143.48incl. VAT
- £149.46 excl. VAT
- £179.35incl. VAT
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Print PageWhatman Puradisc 25 syringe filters
- Nylon membrane
- PP housing
- 25mm diameter
- 0.45µm pore size
- Pack of 50
Features
- 25 mm diameter syringe filter
- Sample volume up to 100 ml
- Low hold-up volume ensures maximum sample recovery
- Glass microfibre option available
- Tube-tip format (optional)
Applications
Depending on material, applications include:
- Biological sample preparation
- UV spectroscopy sample preparation
- HPLC sample preparation
- Enivronmental sample preparation
Nylon membrane filters are hydrophilic, flexible, tear-resistant, and autoclavable. They are resistant to a range of organic solvents and suitable for use with high pH samples. Nylon binds proteins, which makes it unsuitable for protein recovery applications. Suitable for aqueous samples.
Guide to…Syringe Filter Properties
Identifying the filter material most suitable for your application based upon the nature of the liquid or gas you are filtering will ensure the best performance of your filter. Each syringe filter contains a membrane filter of a set diameter and plastic housing with luer connections used for connecting to syringes (or in-line hosing systems).
The membrane filter material has differing chemical and physical properties such as hydrophobicity, flow rate, extractables and protein retention making them suited to differing applications. Once you have selected the most compatible filter material, you will need to consider what pore size is most appropriate for your application and what diameter filter is suitable for your sample volume.
Membrane Material
| Membrane Material | Protein binding | Hydrophilic/Hydrophobic | Chemical resistance | Applications |
| Hydrophilic PTFE | Low | Hydrophilic | High | Purification of HPLC organic and solvent/aqueous solutions |
| Hydrophobic PTFE | Low | Hydrophobic | High | Filtration of solvents |
| PES | Low | Hydrophilic | Low/Medium | Purification of tissue culture solutions, buffers |
| PVDF | Low | Hydrophilic | Medium | Filtration of protein solutions |
| NYL | Medium | Hydrophilic | Medium | Filtration of aqueous and solvent/aqueous mixtures |
| CA | Low | Hydrophilic | Low | Filtration of protein solutions |
| CN | High | Hydrophilic | Low | Filtration of aqueous solutions where protein binding is not a concern and microbioloigical applications. |
| RC | Low | Hydrophilic | Medium | Filtration of aqueous and organic solvents and protein recovery applications. |
Filter diameter
High particulate loaded samples with tend to block the pores in the membrane more quickly so the total volume you can filter through a certain diameter syringe filter will vary greatly. Please take the below figures as a guide only.
- 13mm diameter - for samples <10ml
- 25mm diameter - for samples <100ml
- 33mm diameter - for samples <250ml
For samples that are very particle ladden, it is recommened that you try pre-filtering your sample. Pre-filtering can be achieved with a number of different filters, such as glass microfibre. Some syringe filters come with an in-built pre-filter.
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