Whatman Puradisc 25 syringe filters
PURADISC 25 GD - For prefiltration and where high loading capacity is required; contains Whatman Multigrade glass microfibre.
PURADISC 25 NYL - Nylon media available in 0.2µm and 0.45µm pore sizes. Suitable for the filtration of aqueous samples.
PURADISC 25 AS - Low protein binding polyethersulphone for use with aqueous solutions.
PURADISC 25 TF - Chemically resistant PTFE for organic solvents, especially suitable for HPLC samples.
PURADISC 25 PP - Versatile polypropylene for solvents and aqueous solutions.
PURADISC 25 GF/F - Whatman glass microfibre media ideal for environmental samples.
All the Puradisc 25 devices have polypropylene housings except Puradisc 25 AS which has an acrylic housing. All have easy to use female luer lock/male slip luer connections. 25mm syringe filters feature a minimum volume hold-up but still allow maximum sample recovery of up to typically 100ml volumes.
Features
Identifying the filter material most suitable for your application based upon the nature of the liquid or gas you are filtering will ensure the best performance of your filter. Each syringe filter contains a membrane filter of a set diameter and plastic housing with luer connections used for connecting to syringes (or in-line hosing systems).
The membrane filter material has differing chemical and physical properties such as hydrophobicity, flow rate, extractables and protein retention making them suited to differing applications. Once you have selected the most compatible filter material, you will need to consider what pore size is most appropriate for your application and what diameter filter is suitable for your sample volume.
| Membrane Material | Protein binding | Hydrophilic/Hydrophobic | Chemical resistance | Applications |
| Hydrophilic PTFE | Low | Hydrophilic | High | Purification of HPLC organic and solvent/aqueous solutions |
| Hydrophobic PTFE | Low | Hydrophobic | High | Filtration of solvents |
| PES | Low | Hydrophilic | Low/Medium | Purification of tissue culture solutions, buffers |
| PVDF | Low | Hydrophilic | Medium | Filtration of protein solutions |
| NYL | Medium | Hydrophilic | Medium | Filtration of aqueous and solvent/aqueous mixtures |
| CA | Low | Hydrophilic | Low | Filtration of protein solutions |
| CN | High | Hydrophilic | Low | Filtration of aqueous solutions where protein binding is not a concern and microbioloigical applications. |
| RC | Low | Hydrophilic | Medium | Filtration of aqueous and organic solvents and protein recovery applications. |
High particulate loaded samples with tend to block the pores in the membrane more quickly so the total volume you can filter through a certain diameter syringe filter will vary greatly. Please take the below figures as a guide only.
For samples that are very particle ladden, it is recommened that you try pre-filtering your sample. Pre-filtering can be achieved with a number of different filters, such as glass microfibre. Some syringe filters come with an in-built pre-filter.
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