Whatman Puradisc 13 PTFE 0.2µm Pack of 100

£207.00 excl. VAT
£248.40
incl. VAT

SKU - 1154082

6784-1302

SKU -

1154082

Order now for 7 day delivery

Features

13 mm diameter syringe filter
Sample volume up to 10 ml
Low hold-up volume < 25 µl ensures maximum sample recovery
Glass microfibre option available
Tube-tip format (optional)

Applications

Depending on material, applications include:

Biological sample preparation
UV spectroscopy sample preparation
HPLC sample preparation
Enivronmental sample preparation

Puradisc PTFE syringe filter is a hydrophobic membrane that is chemically stable and inert. It exhibits low protein binding, and its main application is filtration of non-aqueous samples. Prior to filtering of aqueous samples, the membrane must be pre-wetted with a water-miscible organic solvent.

Guide to…Syringe Filter Properties

Identifying the filter material most suitable for your application based upon the nature of the liquid or gas you are filtering will ensure the best performance of your filter. Each syringe filter contains a membrane filter of a set diameter and plastic housing with luer connections used for connecting to syringes (or in-line hosing systems).

The membrane filter material has differing chemical and physical properties such as hydrophobicity, flow rate, extractables and protein retention making them suited to differing applications. Once you have selected the most compatible filter material, you will need to consider what pore size is most appropriate for your application and what diameter filter is suitable for your sample volume.

Membrane Material

Membrane Material	Protein binding	Hydrophilic/Hydrophobic	Chemical resistance	Applications
Hydrophilic PTFE	Low	Hydrophilic	High	Purification of HPLC organic and solvent/aqueous solutions
Hydrophobic PTFE	Low	Hydrophobic	High	Filtration of solvents
PES	Low	Hydrophilic	Low/Medium	Purification of tissue culture solutions, buffers
PVDF	Low	Hydrophilic	Medium	Filtration of protein solutions
NYL	Medium	Hydrophilic	Medium	Filtration of aqueous and solvent/aqueous mixtures
CA	Low	Hydrophilic	Low	Filtration of protein solutions
CN	High	Hydrophilic	Low	Filtration of aqueous solutions where protein binding is not a concern and microbioloigical applications.
RC	Low	Hydrophilic	Medium	Filtration of aqueous and organic solvents and protein recovery applications.

Filter diameter

High particulate loaded samples with tend to block the pores in the membrane more quickly so the total volume you can filter through a certain diameter syringe filter will vary greatly. Please take the below figures as a guide only.

13mm diameter - for samples <10ml
25mm diameter - for samples <100ml
33mm diameter - for samples <250ml

For samples that are very particle ladden, it is recommened that you try pre-filtering your sample. Pre-filtering can be achieved with a number of different filters, such as glass microfibre. Some syringe filters come with an in-built pre-filter.