Water Quality Check in Beer Brewing using LAQUAtwin

Beer is one of the most widely consumed alcoholic drinks and the third most popular drink overall (after water and tea) in the world. It has become a part of the culture of many nations and often associated with social traditions such as festivals and games.1
Brewing is the process of making beer. The four main ingredients of beer are cereal grains (e.g., malted barley, wheat, maize, rice) as starch source, water, flavouring agents (e.g., hops, gruit, herbs, fruits), and yeast. Malted barley and hops are the most commonly used cereal grain and flavouring agent, respectively. In beer brewing, the starch source is mixed with water and converted into a sugary liquid called wort. The wort is subsequently converted into the alcoholic drink by fermentation using yeast.
As beer is mostly water, the composition of brewing water can affect the quality of beer. Brewing water should be clean and odourless. Water can come from two sources—surface water and groundwater.
The former is low in dissolved minerals but higher in organic matter, while the latter is generally higher in dissolved minerals and low in organic matter.2
To determine the mineral composition of water, beer brewers can ask reports from local water companies, send sample water to laboratories, or perform the tests by themselves using proper equipment. The common test parameters are explained below. Based on the results, brewers can make necessary water adjustments to make a great beer.
As water quality check in beer brewing process is a crucial step to achieve exceptional taste and quality of beer, the HORIBA LAQUAtwin pocket meters and LAQUA dissolved oxygen (DO)
handheld meters and dissolved oxygen (DO) electrodes are recommended for beer brewers. Aside from their simple and easy operation, the LAQUAtwin pocket meters have unique flat sensors that can measure sample as low as 0.05 ml in just a few seconds. The LAQUA DO handheld meters are packaged with DO electrodes, which have innovative replaceable DO tips and built-in temperature sensor, in carrying cases for routine measurements in the field. 

Water hardness is defined as the amount of dissolved calcium and magnesium in the water. Calcium is the principal ion that determines water hardness. It can overcome the buffering capacity of malt phosphates, lower the mash pH to acceptable range3 and promote clarity, flavour, and stability in the finished beer.2

The ideal calcium concentration in the brewing water is between 50 and 150 ppm.3 Calcium sulfate (CaSO4), also known as gypsum, or calcium chloride (CaCl2) can be added to increase the amount of calcium ions in water. Magnesium also contributes to water hardness and therefore affects the mash pH, but to a lesser extent than the calcium.2

Alkalinity is the capacity of water to resist changes in pH that would make the water more acidic. It is a measurement of the concentration of all alkaline substances dissolved in the water such as carbonates and bicarbonates which buffer pH in the water by neutralizing acids.
Calcium concentrations must be balanced with low carbonate-bicarbonate levels as they have countering effect on calcium. These ions should be kept to less than 50ppm. Bicarbonates, being
strong alkaline buffers, may raise the pH of the mash to unacceptable levels, if available in large amounts.3

pH is important in various stages of beer brewing—mash, wort, and finished beer. Most municipal water sources are slightly alkaline. When water is combined with grains in a mash tun, the pH of the mixture, called mash, drops compared to the pH of water alone. The mineral composition (i.e., calcium, magnesium, carbonates, and bicarbonate) of the water and natural acidification process in mashing of malted grains drive the mash pH. The ideal mash pH range is 5.2 to 5.5 with preference to the lower end (5.2) for improved enzyme activity leading to optimal conversion of starches to liquid sugars called wort.4 After mashing, the wort is drained, and the grains are washed. This process is called sparging.

As sparging progresses, the pH of wort being run off from the mash increases. The pH of the run-off wort should be below 6 because higher pH will extract tannins, silicates, and other compounds from the grain leading to astringent-off flavours and cloudy, hazy beer.3 The collected wort is transferred to a kettle for boiling. During the boil, calcium phosphate is still precipitated (as long as calcium is still present) and the pH decreases, just like during mashing. A post-boil wort pH of 5.0 to 5.2 should be achieved. This pH range will help extract the best character from hops, maximize the amount of hop break formed, and keep colour pick-up to a minimum.
Another important function of boiling is coagulating the hot break. The optimal pH for break formation is 5.2. If big, fluffy bits of break material in wort are present in early boiling, it is a confirmation that pH is in the right range.5 Hot break must be removed so that the hot wort can be clear.
When yeast is added to the wort, the fermentation begins—sugar turns into alcohol and carbon dioxide. During fermentation, the pH continues to drop because yeast takes in ammonium ions
(strongly basic) and excretes organic acids (includes lactic acid). The yeast strain chosen can affect the final beer pH—most lager beers = pH 4.2 – 4.6, some ales = pH 3.8, sour beers = around
pH 3.0. An optimal pH of less than 4.4 favours faster beer maturation, better beer clarity, better biological stability and more refined beer taste.5

Sodium is a desirable ion in beer for final product flavour complexity. The range is 0-150 ppm Na+. Professional brewers use simple cleaning process or clean-inplace (CIP) techniques to make regular cleaning efficient and effective. Cleaning process / CIP involves circulating cleanser or sanitizer through a spray ball in enclosed equipment. Brewers will clean each time the wort or beer is transferred from a vessel. For example, the kettle must be cleaned after the wort is boiled and sent off through the heat exchanger and into the fermenter. Cleansers with sodium hydroxide (commonly known as caustic soda) as active ingredient are by far the most widely used in breweries.10 Any sodium residue in the equipment after cleaning process / CIP can be checked using an ion selective sensor and meter.

Brewing water from municipal water sources may contain total dissolved solids (TDS) such as minerals, salts, and organic matter. TDS is an indicator of water quality and, as discussed above, mineral composition of water affects mash pH in brewing process. The recommended TDS level in water is 500ppm.6
The amount of TDS in water can be estimated using a TDS meter, which measures the conductivity of solution and converts it to TDS using a factor. Although TDS does not specify the exact mineral composition of water, it can be used in monitoring any changes in the water

Electrical conductivity (EC), often simply referred to as conductivity, is a measure of the ability of an aqueous solution to carry an electric current. Pure water has no conductivity (reading is not zero but very low) because there are no dissolved ions in it. The more ions in solution, the higher the conductivity value. Conductivity measurement of water source in beer brewing gives an indication of the water purity and the baseline measurement before brewer can make any adjustments, like addition of minerals. 

After boiling in the kettle, the hopped wort is devoid of oxygen. It is cooled down rapidly to below 80 °F (27 °C) before oxygenation for better oxygen uptake.
The amount of dissolved oxygen required depends on the yeast strain and the original gravity of the wort. Traditional ale and lager worts were usually not collected higher than 1045 (12°P) and required 6 to 8 ppm dissolved oxygen. With high gravity brewing, original gravities have increased up to 1080 (20°P) and require dissolved wort oxygen levels of 16 ppm
or higher.7
Modern strains of yeast can require as high as 20ppm DO. After oxygenation, yeast is pitched into the wort. Yeast utilizes oxygen to become healthy and to reproduce before fermenting the wort to beer. Oxygen is necessary to produce unsaturated fatty acids and sterols for yeast’s cell walls. A strong cell wall enhances the yeast’ alcohol tolerance. Healthy yeast metabolizes wort
into alcohol and carbon dioxide without giving off-flavours or off-odours.8
Once fermentation is complete, the beer is free from oxygen and must be protected from oxygen exposure to prevent oxidation and staling. It is almost impossible not to introduce oxygen during transfers and packaging (bottling and canning), but the amount should be insufficient for healthy yeast to grow.7 Brewers should be capable of achieving less than 50 ppb (0.05ppm) total in package oxygen.

Malt contains between 4-6 g/kg of potassium, the major part of which is solubilized during mashing; in beer the concentration of potassium lies between 300 – 500 ppm (mg/L). Above 500 ppm,
beer can be salty. Potassium has a purely saline effect. The potassium/calcium ratio affects yeasts flocculation.9

1. Beer – Wikipedia (Accessed on 27 May 2019 at https://en.wikipedia.org/wiki/Beer)
2. Brewing Water (Accessed on 27 May 2019 at https://beerandbrewing.com/brewing-water/)
3. The Power of pH (Accessed on 27 May 2019 at https://byo.com/article/the-power-of-ph/)
4. Mash pH and Why It Matters for All Grain Beer Brewing (Accessed on 27 May 2019 at http://beersmith.com/blog/2015/05/07/mash-ph-and-why-it-matters-forall-grain-beer-brewing/)
5. The Principles of pH (Accessed on 27 May 2019 at https://byo.com/article/the-principles-of-ph/)
6. Reading a Water Report (Accessed on 27 May 2019 at http://howtobrew.com/book/section-3/understanding-the-mash-ph/reading-a-water-report)
7. The Role of Oxygen in Brewing (Accessed on 27 May 2019 at http://www.ibdlearningzone.org.uk/article/show/pdf/494/)
8. Aeration Basics (Accessed on 27 May 2019 at https://www.morebeer.com/articles/Wort_Oxygenation_Aeration)
9. Handbook of Home brewing by William Hardwick, page 142.
10. The Dirt on Brewery Cleaning (Accessed on 27 May 2019 at https://www.morebeer.com/articles/Brewery_Cleaning)