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Whatman SPARTAN syringe filters from Cytiva provide reproducible filtration for ultra-high performance liquid chromatography (UHPLC) and high performance liquid chromatography (HPLC) sample preparation and analysis.
In addition to their use as HPLC syringe filters, SPARTAN filters can be used in capillary gel electrophoresis, protein sample ultrafiltration, UV/VIS analysis, and any application requiring a chemically resistant, low protein binding, hydrophilic membrane in a polycarbonate filter housing.
Identifying the filter material most suitable for your application based upon the nature of the liquid or gas you are filtering will ensure the best performance of your filter. Each syringe filter contains a membrane filter of a set diameter and plastic housing with luer connections used for connecting to syringes (or in-line hosing systems).
The membrane filter material has differing chemical and physical properties such as hydrophobicity, flow rate, extractables and protein retention making them suited to differing applications. Once you have selected the most compatible filter material, you will need to consider what pore size is most appropriate for your application and what diameter filter is suitable for your sample volume.
| Membrane Material | Protein binding | Hydrophilic/Hydrophobic | Chemical resistance | Applications |
| Hydrophilic PTFE | Low | Hydrophilic | High | Purification of HPLC organic and solvent/aqueous solutions |
| Hydrophobic PTFE | Low | Hydrophobic | High | Filtration of solvents |
| PES | Low | Hydrophilic | Low/Medium | Purification of tissue culture solutions, buffers |
| PVDF | Low | Hydrophilic | Medium | Filtration of protein solutions |
| NYL | Medium | Hydrophilic | Medium | Filtration of aqueous and solvent/aqueous mixtures |
| CA | Low | Hydrophilic | Low | Filtration of protein solutions |
| CN | High | Hydrophilic | Low | Filtration of aqueous solutions where protein binding is not a concern and microbioloigical applications. |
| RC | Low | Hydrophilic | Medium | Filtration of aqueous and organic solvents and protein recovery applications. |
High particulate loaded samples with tend to block the pores in the membrane more quickly so the total volume you can filter through a certain diameter syringe filter will vary greatly. Please take the below figures as a guide only.
For samples that are very particle ladden, it is recommened that you try pre-filtering your sample. Pre-filtering can be achieved with a number of different filters, such as glass microfibre. Some syringe filters come with an in-built pre-filter.
The Echotherm™ CO50 unit can accurately maintain the temperature of HPLC columns with chilling as well as heating, so it can be used from 4°C up to 100°C to within 0.1°C. [caption id="attachment_6987" align="aligncenter" width="200"] The Echotherm CO50 Column heater / chiller for HPLC column temperature control[/caption] The CO50 column heater/chiller can also be programmed to enable ...
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