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- Nytran® nylon and Nytran® SuPerCharge
Nytran® nylon and Nytran® SuPerCharge
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£163.00 excl. VAT
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£195.60 inc. VAT
Whatman Nytran nylon membranes are available in two formats. The Nytran N is moderately charged and the Nytran SuPerCharge (SPC) has a very high positive charge.
Nytran Nylon
The Whatman Nytran Nylon membrane is ideal for applications that require a lower charge. It is designed for Southern and Northern blotting as well as colony and plaque lifts and Dot-/Slot-Blots. Nytran N is compatible with isotopic and non-isotopic detection methods.
Nytran N membrane allows for excellent signal-to-noise ratios. The membrane is cast uniformly on both sides of a support matrix, demonstrating excellent symmetry. This gives the membrane the ability to lie flat without curling. Nytran N membrane is a highly consistent membrane with uniform pore size and distribution.It is available in 0.2 µm and 0.45 µm pore sizes for optimal retention of oligos and larger DNA fragments.
Nytran SuPerCharge (SPC)
Nytran SPC nylon membranes have a very high positive charge. Improvements in the manufacturing process result in a membrane with a higher density of nylon per unit area. The increased charge and greater nylon density provide increased binding sites for your samples.
Nytran SPC membranes show a very uniform pore size and pore distribution compared to typical nylon membranes. They are free of surface microvoids
which are common in other membranes. These characteristics lead to greater reproducibility of results across a membrane and from blot to blot.
Nytran SPC membrane is cast uniformly on both sides of a support matrix, demonstrating excellent symmetry. This gives the membrane the ability to lie flat without curling.
With typical manufacturing techniques, increasing positive charge tends to increase background. Nytran SPC membranes are manufactured using a process that allows the combination of high positive charge with low background. Whether using radioactive or non-radioactive detection techniques, Nytran SPC consistently gives high signal with extremely low background.
Nytran binding capacity: >400 µg/cm2; Nytran SuPerCharge: >600µg/cm2
Guide to…Membrane Filter Properties
Identifying the filter material most suitable for your application based upon the nature of the liquid or gas you are filtering will ensure the best performance of your filter. Each membrane filter material has differing chemical and physical properties such as hydrophobicity, flow rate, extractables and protein retention making them suited to differing applications. Once you have selected the most compatible filter material, you will need to consider what pore size is most appropriate.
| Membrane Material | Protein binding | Hydrophilic/Hydrophobic | Chemical resistance | Applications |
| Hydrophilic PTFE | Low | Hydrophilic | High | Purification of HPLC organic and solvent/aqueous solutions |
| Hydrophobic PTFE | Low | Hydrophobic | High | Filtration of solvents |
| PES | Low | Hydrophilic | Low/Medium | Purification of tissue culture solutions, buffers |
| PVDF | Low | Hydrophilic | Medium | Filtration of protein solutions |
| NYL | Medium | Hydrophilic | Medium | Filtration of aqueous and solvent/aqueous mixtures |
| CA | Low | Hydrophilic | Low | Filtration of protein solutions |
| CN | High | Hydrophilic | Low | Filtration of aqueous solutions where protein binding is not a concern and microbioloigical applications. |
| RC | Low | Hydrophilic | Medium | Filtration of aqueous and organic solvents and protein recovery applications. |
Products in this Range
