Eppendorf UVette®

Eppendorf UVette® UV cuvettes

Eppendorf UVette® UV cuvettes. 10mm square cuvettes suitable for most spectrometers Ideal for DNA measurement at 260/280nm and other small samples

About the range

The Eppendorf UVette allows for measurements in the UV/Vis range with transparency from 220nm to 1,600nm with just a typical minimum volume of 50µl.

The UVette allows the user to choose between two optical path lengths (10mm and 2mm).

The path length can be reduced by a factor of x5 without diluting or wasting any sample – simply by turning the UVette 90°. The UVette is suitable for fluorescence assays due to its low auto-fluorescence.

The PCR-clean and protein-free individually sealed variant is ideal for accurate nucleic acid and protein quantification. The UVette is ideally matched for use with the Eppendorf BioPhotometer and Eppendorf BioSpectrometer families. The UVette can be used in other spectrophotometers with the aid of an adapter.

The UVettes are manufactured from crystal clear plastic, which is UV/Vis transparent and features translucency between 220nm and 1,600nm. The openly accessible side walls allow for a visual check of the liquid inside the UVette from all sides.

This enables the user to detect air bubbles, for example, which may alter the test result. Furthermore, the funnel–shaped design of the cuvette base prevents a capillary effect and ensures that even the minimum volume of 50µl constantly remains centered in the measuring area.

Two packaging sizes and purity grades are available:

1. The individually packaged UVettes are PCR-clean & protein-free and are the ideal choice for valuable samples. Samples can be recovered for downstream applications without the risk of degradation or contamination. Lot specific certificates are available on the Eppendorf webpage.
2. The routine pack contains UVettes with Eppendorf certified quality and is the ideal choice for routine applications where many samples need to be screened. It offers safe storage in a reclosable box and convenient access to each cuvette which makes filling easy.

For safe handling, cuvettes should be handled in combination with specific cuvette holders.

One cuvette - two path lengths

The unique shape of the UVette allows sample measurement using two different optical path lengths, which are clearly marked. A rotation of the UVette by 90° provides a choice between the 10mm and the 2mm optical path lengths.

If the sample concentration is too high when measuring using the 10mm light path, the UVette may simply be rotated by 90° and the measurement can be repeated in the 2mm light path.

On the other hand, if the sample concentration is lower than expected, one can easily switch from the 2mm to the 10mm light path. Thus the UVette provides great flexibility by covering measurements across a broad range of concentrations.