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- Eppendorf BioSpectrometer® Fluorescence
Eppendorf BioSpectrometer® Fluorescence
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£6,325.71 excl. VAT
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£9,109.02 inc. VAT
Eppendorf BioSpectrometer® fluorescence
Eppendorf BioSpectrometer® fluorescence 230 V/50?-?60 Hz with GB plug
About the range
The Eppendorf BioSpectrometer fluorescence is an expansion of the Eppendorf BioSpectrometer basic and features an integrated fluorescence module.
UV/Vis assays, fluorescence assays and semi-micro/micro-volume measurements can all be carried out on a single instrument without the need for external control (PC/Laptop).
Eppendorf BioSpectrometer fluorescence can be used to increase the measuring range for biomolecules (nucleic acids and proteins) by a factor of 1,000, compared to UV measurements.
Pre-programmed (modified) methods for Qubit DNA quantification kits (SS, HS, BR) expand the flexibility and functionality of the instrument.
Additionally, the use of the Eppendorf µCuvette G1.0 for fluorescence assays (5µl loading volume) allows for a vast reduction in reagent usage.
Features:
- All Eppendorf BioSpectrometer basic options
- Preprogrammed and freely programmable fluorescence methods
- Preprogrammed (modified) methods for Qubit DNA quantification kits (SS, HS, BR)
- Fluorescence measurement concentration of dsDNA down to 1pg/µl, using Pico Green®
- Fluorescence measurement concentration of BSA down to 0.01ng/µl
- Fluorescence excitation wavelength 470nm, emission wavelengths 520nm and 560nm
- Fluorescence intensity across a range of 0.5nM to 2,000nM fluorescein
- Single consumable (UVette) for UV/Vis and fluorescence assays
- Data transfer via USB interface, Ethernet or via e-mail and direct printout of results
- Data output as *.PNG screenshots, Microsoft® Excel®, or secure PDF files to a standard USB flash drive or via email
Applications:
- Ideal for NGS applications (micro-volume UV/Vis and fluorescence assays in a single instrument)
- dsDNA quantification down to 1pg/µl with common kits, e.g. Pico Green®
- ssDNA quantification down to 10pg/µl
- RNA quantification down to 20pg/µl
- Oligo quantification down to 10pg/µl
- Protein (BSA) quantification down to 0.01ng/µl
- Quantification of GFP-binding proteins
- Growth measurement of GFP-producing bacteria
| Eppendorf BioSpectrometer® fluorescence | |
|---|---|
| Absorption measuring range | 0?A?–?3.0?A (260?nm) |
| BSA concentration range (UV 280 nm) µCuvette® G1.0 | 758 ng/µL – 45,450 ng/µL |
| BSA concentration range (UV 280 nm) UVette® 10 mm | 76 ng/µL – 4,545 ng/µL |
| BSA concentration range (UV 280 nm) UVette® 2 mm | 379 ng/µL – 22,725 ng/µL |
| BSA concentration range (for 1 to 10 mm path length, calculation factor for BSA = 1.515 µg/µL) | 76 ng/µL – 45,450 ng/µL (with fluorescence down to 0.01 ng/µL) |
| Beam receiver fluorescence | Photodiodes |
| Cuvette shaft | 12.5?mm?×?12.5?mm |
| Detector Type | CMOS photodiode array, 1024 pixel |
| Emission wavelength I fluorescence | 520?nm, bandwidth: 15?nm |
| Emission wavelength II fluorescence | 560?nm, bandwidth: 40?nm |
| Excitation wavelength fluorescence | 470?nm, bandwidth: 25?nm |
| Fluorescence measuring range | 0.5?nM?–?2,000?nM fluorescein (emission wavelength 520?nm) |
| Light source absorption | Xenon flash lamp |
| Light source fluorescence | LED |
| Measuring principle absorption | Single-beam absorption spectrophotometer with reference beam |
| Measuring principle fluorescence | Confocal filter fluorometer with reference beam |
| Method memory | >100 method programs |
| Mode absorption | Spectrophotometer |
| Mode fluorescence | Fluorometer |
| Operator guidance language | Spanish, Italian, French, English, German, Japanese |
| Power consumption | Approx. 15?W during operating step Approx. 5?W with dimmed display |
| Random error absorption | ≤0.002 at A?=?0 ≤0.005 (0.5?%) at A?=?1 |
| Random error fluorescence | ±2 % at 1?nM fluorescein |
| Random error wavelength | ≤0.5 nm |
| Random temperature error | – |
| Sample light path length | 8.5 mm |
| Scanning | yes |
| Smallest step size | 1 nm |
| Spectral bandwidth | ≤4 nm |
| Systematic error absorption | – |
| Systematic error wavelength | ±1 nm |
| Systematic temperature error | – |
| Wavelength Selection Increment | 1 nm |
| Wavelength range | 200?nm?–?830?nm |
| Wavelength range absorption | Scan (nm): 200 – 830 at 1 nm increments |
| dsDNA concentration range (UV 260 nm) µCuvette® G1.0 | 25 ng/µL – 1,500 ng/µL |
| dsDNA concentration range (UV 260 nm) UVette® 10 mm | 2.5 ng/µL – 150 ng/µL |
| dsDNA concentration range (UV 260 nm) UVette® 2 mm | 12.5 ng/µL – 750 ng/µL |
| dsDNA concentration range (for 1 to 10 mm path length) | 2.5 ng/µL – 45,450 ng/µL (with fluorescence down to 1 pg/µL) |
| Interfaces | › USB master: for USB stick and thermal printer DPU-S445 › USB slave for connecting to a PC (All functions available without PC) › Serial interface RS-232: for thermal printer DPU-414 › Ethernet interface RJ45: For connecting to a network printer or emailing data outputs directly off instrument |
| Power supply | 100?–?240 V, 50?–?60 Hz |
| Dimensions (W?×?D?×?H) | 295?× 400?× 150?mm?/ 11.6?× 15.7?× 6?in |
| Weight w/o accessories | 5.4 kg |
Servicing Options Available
Have you considered your servicing requirements? Much like a car, scientific instruments may need a bit of care to keep them working reliably and at their most accurate.
The Camlab Service Department have over 60 years experience in scientific instrumentation. We have a number of highly qualified internal and field-based service engineers able to perform a variety of jobs including preventative maintenance servicing and calibration.
Camlab can offer yearly service contracts which include two main types of preventative maintenance/calibration services – the back to base service and an engineer visit to your site. These options are can be offered with differing turn around times depending on the instrument type and your requirements along with loan instruments if available.
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A typical preventative maintenance service includes the following: Inspection and testing of the unit
Dismantling, cleaning and inspection of the key parts
Correction of minor defects including adjustments if necessary
Reassembly and testing
Take post adjustment / cleaning readings
Supply of calibration certificate
Return of the item to you (back to base services)
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Request a quote for your servicing requirements today.
| For more information on this product please click the Request information button below and we will get back to you as soon as we can. |
| Request information |
Alternatively, a consultation visit from one of our highly experienced product specialists is available for this range. Typically our specialist will be able to go through the following aspects with you to help you make the right choices and be able to deliver them on time and and on budget.
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| Request Consultation |
| The links above will take you to our request information page which will forward your enquiry to our team. Please provide as much detail as possible so that we can get back to you with the right information. We normally aim to get back to you same day or within 24 hours on weekdays. If you enquiry is more urgent please call our support team on 01954 233 120 |
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